C-tag TNF: a reporter system to study TNF shedding

A metalloprotease inhibitor blocks shedding of the 80-kD TNF receptor and TNF processing in T lymphocytes

TNF is synthesized as a 26-kD membrane-anchored precursor and is proteolytically processed at the cell surface to yield the mature secreted 17-kD polypeptide. The 80-kD tumor necrosis factor (TNF) receptor (TNFR80) is also proteolytically cleaved at the cell surface (shed), releasing a soluble ligand-binding receptor fragment. Since processing of TNF and TNFR80 occurs concurrently in activated ...

TNF-Induced shedding of TNF receptors in human polymorphonuclear leukocytes: role of the 55-kDa TNF receptor and involvement of a membrane-bound and non-matrix metalloproteinase.

A down-modulation of both the 55-kDa (TNF-R55) and the 75-kDa (TNF-R75) TNF receptors is observed in neutrophils exposed to a variety of stimuli. Proteolytic cleavage of the extracellular region of both receptors (shedding) and, with TNF, internalization of TNF-R55 and shedding of TNF-R75 are the proposed mechanisms. We have characterized the TNF-induced shedding of TNF receptors in neutrophils...

Histamine antagonizes TNF signaling 1 Histamine antagonizes TNF signaling by stimulating TNF receptor shedding from the cell surface and Golgi storage pool

Extracellular sphingomyelinase activity impairs TNF-α-induced endothelial cell death via ADAM17 activation and TNF receptor 1 shedding

ADAM17, a prominent member of the "Disintegrin and Metalloproteinase" (ADAM) family, is an important regulator of endothelial cell proliferation and cell survival. The protease controls vital cellular functions through cleavage of growth factors, cytokines and their receptors including transforming growth factor-alpha (TGF-α), tumor necrosis factor-alpha (TNF-α) and TNF-α receptor 1 (TNFR1). TN...

Targeting TNF-α with a tetravalent mini-antibody TNF-TeAb

Mengyuan LIU*†, Xiangbin WANG†, Changcheng YIN†, Zhong ZHANG†, Qing LIN†, Yongsu ZHEN§ and Hualiang HUANG†‡1 *Faculty of Life Science, Hubei University, 430062 Wuhan, People’s Republic of China, †Beijing ABT Genetic Engineering Technology Co. Ltd., 102206 Beijing, People’s Republic of China, §The Institute of Medicinal Biotechnology, Peking Union Medical College and Chinese Academy of Medical S...